AMPCs_EN_202201_V3

§ Classical Stem Cell Marker CD45+ Molecular Analysis of AMPCs • AMPCs are cultured from leukocytes by reverse engineering • CD34+ cells are leukocyte markers, cells were calculated on day 4 and 5 of culture as total cell counts. Ø Increase in cell count proportions could be attributed to lymphocyte proliferation or AMPCs self- renewal. Ø Self-renewal could be intensified after introduction into the body due to body's signaling proteins. Day0 Day4 Day5 CD45+ 3.8x10 8 4.2x10 8 (110.5%) 4.6x10 8 (121.1%) • Changes in Sca-1 expression against CD45+ expression of leukocytes during the AMPC culture period.