AMPCs_EN_202201_V3

Comparison between Stem Cells Stem Cell Marker Leukocytes (AMPC pre- culture) AMPC (5 day culture) ESC[1,2,3,4] iPSC[5] STAP[6] Oct4 Little to no expression. Shown by RT-PCR. Yes. Significant increase shown by RT-PCR Yes Yes. Significant increase shown by RT-PCR, western blot, DNA microarray. Yes. Significant expression shown by GFP fluorescence and Q-PCR. Nanog Little to no expression. Shown by RT-PCR. Yes. Slight increase shown by RT-PCR Yes Yes. Significant increase shown by RT-PCR, western blot, DNA microarray, immunocytochemistry. Yes. Significant expression shown by Q-PCR. Sox2 Little to no expression. Shown by RT-PCR. Yes. Significant increase shown by RT-PCR Yes Yes. Significant increase shown by RT-PCR, western blot, DNA microarray. Yes. Significant expression shown by Q-PCR. SSEA-1 Yes. Slight expression shown by flow cytometry. Yes. Though expression decreased from pre-culture shown by flow cytometry. No. Increases after differentiation. No. Shown via immunocytochemistry. Yes. Shown via immunostaining SSEA-3 Little to no expression. Shown by flow cytometry. Little to no expression. Shown by flow cytometry. Yes Present. Shown via immunocytochemistry. N/A SSEA-4 Little to no expression. Shown by flow cytometry. Little to no expression. Shown by flow cytometry. Yes Present. Shown via immunocytochemistry. N/A TRA-1-60 Little to no expression. Shown by flow cytometry. Little to no expression. Shown by flow cytometer. Yes Present. Shown via immunocytochemistry. N/A TRA-1-81 Little to no expression. Shown by flow cytometry. Little to no expression. Shown by flow cytometry. Yes Present. Shown via immunocytochemistry. N/A Lin28 Little to no expression. Shown by RT-PCR. Yes. Slight increase shown by RT-PCR Yes Yes. Shown by western blot. N/A Klf4 Little to no expression. Shown by RT-PCR. Yes. Slight increase shown by RT-PCR Yes Yes. Slight increase shown by Q-PCR, RT- PCR, western blot. N/A c-MYC N/A N/A Yes Yes. Slight increase shown by Q-PCR, RT- PCR, western blot. N/A