AMPCs_EN_202201_V3

Comparison between Stem Cells Stem Cell Marker AMPC (5 day culture) iPSC[5] Oct4 Yes. Significant increase shown by RT-PCR Yes. Significant increase shown by RT-PCR, western blot, DNA microarray. Nanog Yes. Slight increase shown by RT-PCR Yes. Significant increase shown by RT-PCR, western blot, DNA microarray, immunocytochemistry. Sox2 Yes. Significant increase shown by RT-PCR Yes. Significant increase shown by RT-PCR, western blot, DNA microarray. SSEA-1 Yes. Though expression decreased from pre- culture shown by flow cytometry. No. Shown via immunocytochemistry. SSEA-3 Little to no expression. Shown by flow cytometry. Present. Shown via immunocytochemistry. SSEA-4 Little to no expression. Shown by flow cytometry. Present. Shown via immunocytochemistry. TRA-1-60 Little to no expression. Shown by flow cytometer. Present. Shown via immunocytochemistry. TRA-1-81 Little to no expression. Shown by flow cytometry. Present. Shown via immunocytochemistry. Lin28 Yes. Slight increase shown by RT-PCR Yes. Shown by western blot. Klf4 Yes. Slight increase shown by RT-PCR Yes. Slight increase shown by Q-PCR, RT-PCR, western blot. c-MYC N/A Yes. Slight increase shown by Q-PCR, RT-PCR, western blot.